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*Casio Ctk 481 Price
*Casio Ctk 2550 Ppk
*Casio Ctk 2550 Manual
*No matter who (or where) you are, Casio’s CTK-2550 portable keyboard opens up a whole world of music with 61 full-size keys, 400 high-quality Tones, and 150 built-in Rhythms including the all-new Dance Music Mode.
*Rockville Double X Braced Keyboard Stand w/Push Lock For Casio CTK-2550. Musician’s Gear 76-Key Keyboard Gig Bag.
*Check out the Casio CTK-2550 Digital keyboard! This rocking’ Little keyboard comes with a ton of amazing onboard sounds and great playing features. Even if you’ve never touch a keyboard before, the CTK-2550 is a great instrument for you. It’s got a cool 3-step learning system, Plus 400 Inspiring sounds to.
*The Casio CTK-2550 PPK comes with everything you need right out of the box including a pair of closed-cup headphones, X-style stand, and power supply. No matter who (or where) you are, Casio’s CTK-2550 portable keyboard opens up a whole world of music with 61 full-size keys, 400 high-quality Tones, and 150 built-in Rhythms including the all.Piano-style keyboard
Casio’s CTK-2550 is one of the greatest Portable Keyboards with tech features expected from professional products.
An easy-to-play keyboard with an appearance resembling that of an acoustic piano.Digital effects
Use effects to add reverb for more depth and breadth in your music.Add to the fun by linking the keyboard with the downloaded Chordana Play app in your smart device.
[Availability] LK-265, CTK-3500, CTK-2550, CTK-2500

*CTK Series keyboards do not offer Key Lighting.Expand the keyboard’s store of built-in songs
Use an audio cable to transfer 50 built-in songs from the app as well as songs downloaded as MIDI files and save them in the keyboard. Free windows slots game downloads. You can then use the saved song data with the keyboard accompaniment function to enjoy the Step Up Lesson feature.
*Songs stored in the keyboard may sound different from original built-in songs.LIGHTLY ROWLONG LONG AGOSAKURA SAKURAAULD LANG SYNEMICHAEL ROW THE BOAT ASHOREHOME ON THE RANGESANTA LUCIAJOSHUA FOUGHT THE BATTLE OF JERICHOTHE MUFFIN MANIF YOU’RE HAPPY AND YOU KNOW IT, CLAP YOUR HANDSHOUSE OF THE RISING SUNSHE WORE A YELLOW RIBBONMY OLD KENTUCKY HOMESZLA DZIEWECZKATROIKAWALTZING MATILDAON THE BRIDGE OF AVIGNONCAMPTOWN RACESJEANNIE WITH THE LIGHT BROWN HAIRJAMAICA FAREWELLJE TE VEUXSONATA op.13 ’PATHÉTIQUE’ 2nd Mov.HEIDENRÖSLEINHABANERA FROM ’CARMEN’BRINDISI FROM ’LA TRAVIATA’Play the keyboard while linked with the app
A piano roll showing the music score and finger timing is displayed on the mobile device’s app screen.
* Mobile device and audio cable sold separately.
Another fun feature is the ability to take 3-step lessons on the smart device itself using the virtual keyboard that appears on the screen. You can confirm the chords, melody and finger timing visually on the screen, while also using the scoring function to help you master the song. The 50 built-in app songs and your downloaded MIDI file songs are all available for use in your lessons.Easy dance tune playback - Just touching the keys is all it takes to play dance music expressing a DJ mood. Or you can use Build-up Play to apply sound effects characteristic of dance music for even more fun.
[Availability] CTK-3500, CTK-2550, CTK-2500, CTK-1550, CTK-1500, LK-265, LK-190, LK-136, LK-135
Pressing the indicated black keys livens up the music with six dramatic sound effects!Lesson Lite
In the Lesson Lite, notes appearance on the display screen. Play notes on the keyboard in accordance with the instructions provided by Voice Fingering Guide.Voice Fingering Guide
If the keyboard senses you are having problems, it will help you by calling out finger numbers in a simulated human voice.Lesson Part Select(right hand, left hand, both hands)Metronome60 Song Bank Tunes
Built-in songs cover a wide range of genres for lesson play and for your listening enjoyment.Designed and engineered to help you sound your best!400 high-quality tones
A wide selection of musical instrument tones supports play of songs from various genres.100 rhythms and Auto Accompaniments for Latin music and other styles from around the world (100 rhythms including 46 ethnic rhythms and 10 patterns for piano play)
An advanced Auto Accompaniment feature provides you with access to a large collection of ethnic rhythms from around the world, with a special emphasis on Latin rhythms. In addition to Bossa Nova, Samba, and other basic rhythms, you also get Cumbia, Reggaeton, Calypso, and more!One Touch Preset (100 presets)
One Touch Preset automatically sets up the keyboard to match the currently selected rhythm.
The Portable piano becomes so popular lately because of its great features and technology. Not only have the richest sound, but also have so many features that easier you make a wonderful piece of music. That is why so many brands digitally built the digital piano.
Speaking of a portable digital piano brand, now we have the most two popular pianos in the market. 7 slot society. They are the CTK 2550 series from Casio and PSR F51 series from Yamaha. You may find that Casio and Yamaha are the familiar brands of the piano.
So, now let’s take a look over them below so that you can decide which one has the most suitable for you.Casio CTK 2550
Casio CTK 2550 is a kind of portable piano that is built specifically to save your time. You can bring it everywhere and whenever you want, but you can still make great music from this piano. It comes with a standard pack and premium pack that you can choose which one has suitable for you the most.
Casio CTK 2550 comes with 61 full-size keys with a bit downer in a good way. This makes the sound better to hear without any resemble on its. The sound while you play the acoustic sound will be better than any other pianos. Besides, without the weighted features makes this piano so lighter to bring.
Casio CTK 2550 offers 400 built-in tones that make it easier for you to create great music. It is so easy to use even for the beginner, and with these variations, it makes your music will come differently from others. Your song will come uniquely and that is perfect even for the beginner.Casio CTK 2550 Yamaha PSR F51Product Dimensions12.09 x 37.24 x 3.62 inches39.1 x 14.9 x 6.3 inches
Shipping Weight7.28 pounds10.8 poundsBest offerCheck priceCheck price
Casio CTK 2550 comes with the step-up lesson system that perfect for the beginner. You can start to learn everything on this portable piano. You will find the lesson an interesting and fun way so that you will more understand the music. Not only teach some lessons but you can also discover so many kinds of pieces of music.
The LCDs on the Casio CTK 2550 will help you to read everything there. You will find the easier way to learn the piano if you are a beginner. But, if you are a professional one you will see that this piano can improve your skill with the feedback from its.
Casio CTK 2550 offers the dance music features with the tune playback technology. not only can make dance music, but you can also remix them into whatever you like. It is so easy to use with only one tap on the button and you can mix them perfectly with the hall, reverb, or dance music sound effects. Read also: Yamaha CP88 Vs Nord Piano.
The dimension from the Casio CTK 2550 comes in a weight light size at only about 12.09 x 37.24 x 3.62 inches and 7.28 pounds of weight. The price also comes at a reasonable rate starts from US$109 on Amazon. It comes with a USB connection, microphone, and sustain pedal jack.Yamaha PSR F51
Yamaha PSR F51 is a kind of portable piano that is made for the beginner. It is a so compact, lightweight, and affordable piano that perfect for everyone not only a beginner. The feature comes in the easier way to learn so that as a beginner you can be a fast learner. You will get a different experience while improve your skill by using this piano.
Yamaha PSR F51 offers the 61 keys with more than 120 sounds already established there. The key comes with a full-size type so that it is so easy to use with your intuitive feeling. The panel comes more quickly so that you can understand what inside there.
Yamaha PSR F51 comes with a color-coded panel that will help you a lot to learn everything there. The digital display will help you to correct your play so that it is perfect for your music improvements. You can simply operate by choosing the instrument voice and rhythm as you want.
The dual voice on the Yamaha PSR F51 brings the combination of the instrument so that you can easily mix your sound. You can create a simple or even an amazing piece of music in a unique way. You will find so many types of sounds while you explore this piano.
Yamaha PSR F51 offers the rich and powerful sound from the built-in metronome sounds on its. The sound will come realistically, and the combination between the rhythm and instrument voice comes in a flawless sound to hear. With the 114 styles of music, you can simply choose your favorite or even mix them.
Yamaha PSR F51 comes with the AC adaptor, headphone output connector, the AA battery types, and Ni-MH rechargeable battery type with power consumption up to six volts. The preset music comes in up to 30 preset songs so that you can makes as much as you want by using this piano.
The dimension of the Yamaha PSR F51 comes in a lightweight size of about 99.8 x 37.6 x 16.2 cm and 3.4 kilograms. The price also comes at a reasonable rate starts from US$111 on Amazon.Casio CTK 2550 Vs Yamaha PSR F51- Dance music Mode w/50 built-in dance music rhythms- 48 note polyphony- A starter keyboard for anyone who wants to enjoy playing music- The PSR-F51 is incredibly easy to use with its color-coded, intuitive control panel.- Simply select a voice, choose a rhythm and start. It’s as simple as 1, 2, 3 !- With 120 Voices & 114 Styles, the PSR-F51 is amazingly versatileWhich One Do You Prefer?
After getting the review above, we jump to the conclusion that Casio CTK 2550 is a better way to pick than the Yamaha PSR F51. As comes from the features, it comes in better technology and features than the Yamaha PSR F51 at almost the same price.Casio Ctk 481 Price
As come from the dimension, Casio CTK 2550 comes in a lightweight way than the Yamaha PSR F51. It is a more compact weight than Yamaha with only about 7.28 pounds of weight. Besides, it has more than 400 built-in tones that amazing to make any type of music as much as you want.Casio Ctk 2550 Ppk
On the other hand, Yamaha PSR F51 only offers about 120 tones, so that makes this piano lack of rich sounds comparing with the Casio CTK 2550 that has so many tones. That makes Casio pianos is very affordable to buy.Casio Ctk 2550 ManualRelated Posts:
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In an hourlong Instagram Live video Tuesday night, Rep. Alexandria Ocasio-Cortez, D-N.Y., described her personal experience last week when a violent mob of pro-Trump extremists breached the. And Ocasio-Cortez, in a nod to the video, posted footage of herself on Twitter Friday morning, dancing to Edwin Starr’s “War” as she prepared to enter her new Capitol Hill office, along with the words, “I hear the GOP thinks women dancing are scandalous. Wait till they find out Congresswomen dance too!”.What is the ’Green New Deal’ proposal drafted by incoming Rep. Alexandria Ocasio-Cortez?
Ocasio-Cortez has been calling for the creation of ’Green New Deal’ legislation, which would eliminate much of the United States’ fossil-fuel consumption. So far, Sens. Bernie Sanders, I-Vt., and Cory Booker, D-N.J., and 40 House Democrats have said they support the proposal, but incoming House Democratic leadership has put it on pause for now.
An old video of now-Rep. Alexandria Ocasio-Cortez playfully recreating a dance scene from “The Breakfast Club” was resurfaced anonymously on Twitter last week -- resulting in a slew of misleading stories claiming conservatives were outraged over it, despite virtually no supporting evidence.
The 2010 footage of Ocasio-Cortez dancing comes from her days at Boston University. It went viral, for sure. But The New York Times, GQ, Newsweek and Ocasio-Cortez, among others, were quick to falsely claim that conservatives were somehow upset by the harmless video.
“It caused a social media stir, with the mainstream media and liberals lining up to cheer it and condemn conservatives for being offended by it. The only problem: Conservatives were not actually offended by it,” Media Research Center’s Clay Waters wrote.
The Times published a story headlined, “Alexandria Ocasio-Cortez Dancing Video Was Meant as a Smear, but It Backfired.” It doesn’t feature a single example of anyone being offended with the exception of an anonymous Twitter account that first drew attention to the video saying it showed ’America’s favorite commie know-it-all acting like the clueless nitwit she is.’ However, the Times managed to find examples of Ocasio-Cortez supporters condemning conservatives for being critical of the video.
Waters called it the “latest knee-jerk liberal mockery of conservatives,” who “stand accused on absolutely no evidence of being offended by something.”
Conservative strategist Chris Barron told Fox News that the mainstream media is “so desperate to make conservatives look bad that they will invent a controversy when there isn’t one,” with the Ocasio-Cortez video the latest example.
“If you want to look up the very definition of fake news, look no further than the faux controversy over the AOC dancing video. Literally, every single conservative I saw comment on the video said it actually made her more likable, and yet our shameless mainstream media spends days pushing the narrative that conservatives attacked her,” Barron said. “Must be nice being a liberal.”
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Perhaps the most overblown example was published by Newsweek, which wrote that “Conservatives mock Alexandria Ocasio-Cortez for college dancing video, everyone else thinks it’s adorable,” while not citing any ’conservatives’ beyond the original anonymous Twitter account. Media watchdogs pounced on Newsweek for the misleading headline.Ocasio Cortez Video Dance College
NowThis likewise posted a video claiming conservatives tried to ’shame’ Ocasio-Cortez with the clip, but mostly cited tweets praising her cool dance moves -- with not much in the way of criticism beyond the original anonymous tweet.
The social media-savvy Ocasio-Cortez used the viral video as an opportunity to knock political rivals, tweeting “I hear the GOP thinks women dancing are scandalous,” with footage of her dancing in the halls of Congress. While Ocasio-Cortez didn’t provide examples of Republicans claiming dancing is “scandalous,” her new video has been viewed nearly 20 million times.Ocasio-cortez Video South Accent Youtube
The Daily Caller’s Joe Simonson wrote that the dancing story “served as another reminder of how desperate the media wishes to portray the GOP and its voters as something very different from what they truly are.”
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*How To Put Two Quotes Next To Each Other
*Two Quotes Next To Each Other
This line may be one of the most frequently quoted, and frequently misunderstood, lines in all of Shakespeare. Though Juliet is standing on her balcony, unaware of Romeo below her, the line doesn’t mean she’s asking where he is. Wherefore means why, so Juliet is asking why Romeo is who he is—namely, a Montague, and therefore her sworn enemy.My only love sprung from my only hate
Too early seen unknown, and known too late! (I.v.)
How To Put Two Quotes Next To Each Other
Juliet complains that she saw Romeo and fell in love with him “too early,” before she knew he was her enemy. Almost everything happens to Juliet too early. She is told to prepare herself for marriage before she is ready, she marries Romeo before she can get her parents’ permission, her marriage to Paris is moved forward twice, and Romeo arrives at her tomb before she has time to wake up.
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Nor arm nor face nor any other part
Belonging to a man. Oh, be some other name!
What’s in a name? That which we call a rose
By any other word would smell as sweet. (II.ii)

Where should I put the comma and the quotes in such a sentence? The comments were significantly focused on my being “engaging, patient and extremely helpful,” “very coherent and easy-to-understand,” or ’clear and straight to the point’. Separate each quote with quotation marks as the two different quotes may well have been said by the originator at different times and circumstances. There was a four year old child whose next door neighbor was an elderly gentleman who had recently lost his wife. Upon seeing the man cry, the little boy went into the old gentleman’s yard, climbed onto his lap, and just sat there. When his Mother asked what he had said to the neighbor, the little boy said, “Nothing, I just helped him cry.²”.
Juliet is practical. She argues that Romeo’s name is not a part of his body, so it’s not an essential part of him. The audience might think of Romeo’s genitals when she lists “any other part / Belonging to a man,” especially since Juliet’s language is often physical and erotic. But here she is also philosophical, exploring language’s relationship to how we experience reality. Casino full movie 1995 youtube.My bounty is as boundless as the sea,
My love as deep; the more I give to thee,
The more I have, for both are infinite. (II.ii.)

Like Romeo, Juliet sees love as a kind of freedom, “boundless” and “infinite.” The suggestion that Juliet will “give” her “bounty” to Romeo is the most explicitly erotic moment in their conversation so far. Throughout the play, Juliet takes the lead in the sexual side of their relationship.Hist, Romeo, hist! O for a falconer’s voice
To lure this tassel-gentle back again. (II.ii)

Earlier in this scene, Romeo imagined himself with “light wings.” In these lines, Juliet picks up on this image to picture Romeo as a tame falcon and herself as a falconer. Juliet’s image suggests she feels she has power over him. The fact that she takes Romeo’s metaphor and bends it to her own purposes also suggests her sense of power in their relationship.Come, gentle night, come, loving black-browed night,
Give me my Romeo, and when I shall die,
Take him and cut him out in little stars. (III.ii.)

In these lines, Juliet looks forward to her wedding night in explicitly sexual terms. “Die” was Elizabethan slang for orgasm, which turns her image of Romeo as a sky full of stars into a metaphor for sexual climax. The violence of the image also reminds us that in Romeo and Juliet, sex and violence are never far apart.So tedious is this day
As is the night before some festival
To an impatient child that hath new robes
And may not wear them. (III.ii.)

As Juliet expresses her sexual maturity by longing for her wedding night, she compares herself to an “impatient child.” This serves to remind the audience that Juliet is not yet fourteen. Throughout the play, Juliet matures and reaches major life events too early, which foreshadows that she will also die much too young.Two Quotes Next To Each OtherO God, I have an ill-divining soul!
Methinks I see thee now, thou art so low,
As one dead in the bottom of a tomb. (III.v.)

Just before Romeo and Juliet met, Romeo had an intuition that his life was about to take a tragic turn. In this scene, which is the lovers’ last scene alive together, it is Juliet’s turn to foresee their tragic fate. These two moments bookend the lovers’ relationship and show that from beginning to end, Romeo and Juliet share a single fate and experience it together.I will kiss thy lips.
Haply some poison yet doth hang on them
To make me die with a restorative. (V.iii.)

These are Juliet’s last words. She imagines the poison that has killed Romeo as a “restorative,” a medicine that can put an end to her suffering. One of the play’s major themes is the inseparability of good and evil, love and hate, poison and cure. Juliet’s death is tragic, but she also celebrates it as a way of escaping a life without her beloved.
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We spent two short days in Greece. One full day in Athens and another day taking a trip out the the close islands. We spent a majority of our Europe trip in Italy, but wanted to get a feel for Greece too. I definitely want to come back to the main islands, Mykonos and Santorini.
Athens
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Wow, it’s hard to put into words seeing some of this ancient history!
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Use your guidebook to do a self-guided walk through the ruins. We walked here from our hotel and spent at least five-six hours walking through the ruins. The area is huge! We didn’t grab lunch until 4 PM.
Visit Restaurant Row
Of course, you have to try the souvlaki. Keep in mind, if you sit down, they charge you more of your meal vs. doing take away. We didn’t know this until the bill arrived. Gplots heatmap. It was nice to rest our feet tho!
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Yum! That’s all I have to say. We loved it so much, we make it for pot luck parties here.
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18.1heatmap.2 function from gplots package A heatmap is a graphical representation of data where the values are represented with colors. The heatmap.2function from the gplotspackage allows to produce highly customizable heatmaps. # install gplots. A heat map is a false color image (basically image (t (x))) with a dendrogram added to the left side and/or to the top. Typically, reordering of the rows and columns according to some set of values (row. Demonstrate the effect of row and column dendrogram options heatmap.2(x) ## default - dendrogram plotted and reordering done. Heatmap.2(x, dendrogram=’none’) ## no dendrogram plotted, but. Gplots Introduction. This repo is to keep the gplots package alive. I don’t plan to develop new features, but if you’ll send pull requests I’m willing to review them. You can see the most recent. Various R programming tools for plotting data, including: - calculating and plotting locally smoothed summary function as (’bandplot’, ’wapply’), - enhanced versions. Gplots heatmap.
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Get great deals on Casino (1995 film) DVDs & Blu-ray Discs. Expand your home video library from a huge online selection of movies at eBay.com. Fast & Free shipping on many items! Movies & TV New Releases Best Sellers Deals Blu-ray 4K Ultra HD TV Shows Kids & Family Anime All Genres Prime Video Your Video Library 1-16 of 323 results for Movies & TV: ’casino movie. Original release and VHS showed a mirror with lines of cocaine in the hotel drawer of the Senator / Congressman along with the money chips. In the later DVD release the cocaine is no longer there. The version shown in Swedish cinemas in 1996 removed 43 meters of.
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Martin Scorsese’s fascinating new film ’Casino’ knows a lot about the Mafia’s relationship with Las Vegas. It’s based on a book by Nicholas Pileggi, who had full access to a man who once ran four casinos for the mob, and whose true story inspires the movie’s plot.
18.1heatmap.2 function from gplots package A heatmap is a graphical representation of data where the values are represented with colors. The heatmap.2function from the gplotspackage allows to produce highly customizable heatmaps. # install gplots. A heat map is a false color image (basically image (t (x))) with a dendrogram added to the left side and/or to the top. Typically, reordering of the rows and columns according to some set of values (row. Demonstrate the effect of row and column dendrogram options heatmap.2(x) ## default - dendrogram plotted and reordering done. Heatmap.2(x, dendrogram=’none’) ## no dendrogram plotted, but. Gplots Introduction. This repo is to keep the gplots package alive. I don’t plan to develop new features, but if you’ll send pull requests I’m willing to review them. You can see the most recent. Various R programming tools for plotting data, including: - calculating and plotting locally smoothed summary function as (’bandplot’, ’wapply’), - enhanced versions. Gplots heatmap.
Plot legend matlab. Like ’The Godfather,’ it makes us feel like eavesdroppers in a secret place.
The movie opens with a car bombing, and the figure of Sam ’Ace’ Rothstein floating through the air. The movie explains how such a thing came to happen to him. The first hour plays like a documentary; there’s a narration, by Rothstein (Robert De Niro) and others, explaining how the mob skimmed millions out of the casinos.
It’s an interesting process. Assuming you could steal 25 percent of the slot-machine take - what would you do with tons of coins? How would you convert them into bills that could be stuffed into the weekly suitcase for delivery to the mob in Kansas City? ’Casino’ knows. It also knows how to skim from the other games, and from food service and the gift shops. And it knows about how casinos don’t like to be stolen from.
There’s an incident where a man is cheating at blackjack, and a couple of security guys sidle up to him and jab him with a stun gun.
He collapses, the security guys call for medical attention, and hurry him away to a little room where they pound on his fingers with a mallet and he agrees that he made a very bad mistake.
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Rothstein, based on the real-life figure of Frank (Lefty) Rosenthal, starts life as a sports oddsmaker in Chicago, attracts the attention of the mob because of his genius with numbers and is assigned to run casinos because he looks like an efficient businessman who will encourage the Vegas goose to continue laying its golden eggs. He is a man who detests unnecessary trouble. One day, however, trouble finds him, in the person of Ginger McKenna (Sharon Stone), a high-priced call girl.
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A Whisker Away A third song called “Usotsuki” by J-pop duo Yorushika will be featured as the ending theme in the upcoming Netflix movie A Whisker Away (Japanese title: Nakitai watashi wa neko wo. The story of the song itself seems to tell of a girl dreaming of growing up and emerging into society, lost and consumed by her thoughts. Nowadays it’s hard to tell what exactly was coming through Yosui Inoue’s mind as he made this piece of music, but still, I find it to be fascinating and beautiful in its uniqueness, melody, and the legacy it. 1 Background 2 Succeeding versions 3 Lyrics 4 Derivatives 5 Other media appearances 5.1 Discography 5.2 Concerts 6 Gallery 7 External links 7.1 Official 7.2 Unofficial ’Usotsuki wa Koi no Hajimari’ is an original demo song by 40mP featuring Luo Tianyi V4 Japanese. This song exceeded 100,000 views on bilibili. It reached 1 million views on YouTube in February 2019. This song was featured in the. Download free slots game for windows 10.
* Artist: Aisha (Japan)(あいしゃ)
*Song: Usotsuki LIAR
*Album: Love sickEnglish, Japanese/Romanization Usotsuki LIAR ✕Usotsuki LIARI don’t want you back Anoko ga ii naraI don’t want you back BABY matteKoe ga furue teru motokare Atashi o mitsumeteImasara Saigo no chansu ageteAnata ni usotsukareteMo You’re such a liar Usotsuki LIARI don’t want you back Anoko ga ii naraI don’t want you backDare na no Hey Hello?Futari no kisushinKono mama de ita no?Kimi no tame niMoyashite ku tegamiUsotsuki LyricsMo You’re such a liarUsotsuki Song LyricsUsotsuki LIARI don’t want you back Anoko ga ii naraI don’t want you back Uso bakka tsuite majidasaiOmae no denwa ni mo denai Saru mono oubekarazu Usotsuki LIARI don’t want you back Usotsuki SongAnoko ga ii naraI don’t want you backUsotsuki Song✕1.Usotsuki LIAR2.愛にいくよ (Ai Ni Iku Yo)3.Shy Demoiiyo
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*Steel Casing Pipe Sizes
*A Casing Is A Steel Pipe Thickness
*A Casing Is A Steel Pipe Fittings
Slots game download free. Casing pipe is a type of steel pipe that is typically used to enclose and surround a carrier pipe, which is installed through the center of this pipe. Steel casing pipe is most often used in underground construction projects to encase or protect utility lines from being damaged. Casing Pipe: Petroleum casing pipes are steel pipes used to support the walls of oil and gas wells to ensure the normal operation of the entire oil well after drilling and completion. Each well uses several layers of casing according to different drilling depths and geological conditions. Steel Pipe Casing: Steel pipe casing shall be manufactured from steel conforming to ASTM Grade 2 as amended to date, with a minimum yield strength of 35,000 psi before cold forming. (1) Pipe may be straight seam or spiral welded. A protective coat will not be required. Casing pipe is a type of steel pipe that is typically used to enclose and surround a carrier pipe, which is installed through the center of this pipe. Steel casing pipe is most often used in underground construction projects to encase or protect utility lines from. (Redirected from Steel Casing Pipe)
Steel casing pipe, also known as encasement pipe, is most commonly used in underground construction to protect utility lines of various types from getting damaged. Such damage might occur due to the elements of nature or human activity. Steel Casing Pipe Sizes
Steel casing pipe is used in different types of horizontal underground boring, where the pipe is jacked into an augered hole in segments and then connected together by welding or by threaded and coupled ends, or other proprietary pipe connectors such as the interference-fit Permalok® interlocking push-on joint. The steel casing pipe can also be set up and welded into a ’ribbon’ and then directionally pulled through a previously drilled hole under highways, railroads, lakes and rivers. [1]Uses[edit]18’ ductile iron gravity sewer pipe being installed within 30’ steel encasement
Steel casing pipe protects one or many of various types of utilities such as water mains, gas pipes, electrical power cables, fiber optic cables, etc. The utility lines that are run through the steel casing pipe are most commonly mounted and spaced within the steel casing pipe by using ’casing spacers’ that are made of various materials, including stainless steel or carbon steel and the more economical plastic versions. The ends of a steel casing pipe ’run’ are normally sealed with ’casing end seals’, which can be of the ’pull-on’ or ’wrap-around’ rubber varieties. Steel casing pipe is also used in the construction of deep foundations.Specification[edit]
Steel casing pipe generally has no specific specifications, other than the need for the material to be extremely straight and round. In some areas A.S.T.M. specifications may be required by project engineers. The specification most commonly called for is A.S.T.M. 139 Grade B. This specification gives parameters for minimum yield and tensile strength of the steel pipe being used for casing, and tolerances of straightness and concentricity.
Steel casing pipe is often specified as ASTM A-252 which is a structural grade material that does not require hydrostatic testing and the inspection requirements are not stringent and it usually costs less than other grades such as A-53, A-139 or API 5L. Used natural gas line pipe is also used as casing on many projects because it is often reclaimed in very good condition and can offer a significant cost savings when compared to new steel pipe. Used pipe is most likely to not have any testing data associated with it and is generally used when the only required specification is a given diameter and wall thickness of steel casing pipe.See also[edit]References[edit]A Casing Is A Steel Pipe Thickness
*^’Steel Casing Pipe (Encasement Pipe)’. http://www.allsteelpipe.com. Retrieved 2013-05-09.External link in |website= (help)A Casing Is A Steel Pipe FittingsRetrieved from ’https://en.wikipedia.org/w/index.php?title=Steel_casing_pipe&oldid=913894543’
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*Ongoing Allocation Refund
*Allocation Of Refund Form
*Allocation Refund Direct Service
Refund claims/applications must be filed within two years from the close of the taxable quarter when the sales were made. Once the claim/application is filed by the taxpayer, the BIR office where the claim was filed is required to act on the refund claim within a mandated 120-day period. Spouse Allocation. Deposit of refund to only one account. If you want your refund deposited to only one account, don’t complete this form. Instead, request direct deposit on your tax return. Account must be. An easy allocation method is to divide the year’s interest by 12, and then multiply the figure by the number of months you lived in each state. Related Information: How do I file a part-year state return? If you file a paper return, use IRS’ Form 8888, Allocation of Refund (Including Savings Bond Purchases) PDF, to split your refund among two or three different accounts. Form 8888 is not required if you want IRS to direct deposit your refund into a single account; you can use the direct deposit line on Forms 1040, 1040-A or 1040-EZ.By Sally P. Schreiber, J.D.

7 slot society of america. In Rev. Rul. 2019-11, issued Friday, the IRS addressed how the long-standing tax benefit rule interacts with the new $10,000 limit on deductions of state and local taxes to determine the portion of any state or local tax refund that must be included on the taxpayer’s federal income tax return.Ongoing Allocation Refund
Sec. 164 generally provides an itemized deduction for certain taxes paid or accrued during the tax year. However, Sec. 164(b)(6), as added by the law known as the Tax Cuts and Jobs Act (TCJA), P.L. 115-97, limits an individual’s deduction for the aggregate amount of state and local taxes paid during the calendar year to $10,000 ($5,000 in the case of a married individual filing a separate return). Sec. 111(a), which partially codifies the tax benefit rule, excludes from gross income amounts attributable to the recovery during the tax year of any amount deducted in any prior year to the extent the amount did not reduce the amount of tax imposed by Chapter 1 of the Code.
Taxpayers who itemize deductions and who paid state and local taxes in excess of the state and local tax deduction limit may not be required to include the entire state or local tax refund in income in the following year. According to the IRS, a key part of that determination is calculating the amount the taxpayer would have deducted had the taxpayer only paid the actual state and local tax liability, with no refund and no balance due.
The IRS therefore ruled that if a taxpayer received a tax benefit from deducting state or local taxes in a prior tax year and the taxpayer recovers all or a portion of those taxes in the current tax year, the taxpayer must include in gross income the lesser of:
*The difference between the taxpayer’s total itemized deductions taken in the prior year and the amount of itemized deductions the taxpayer would have taken in the prior year had the taxpayer paid the proper amount of state and local tax; or
*The difference between the taxpayer’s itemized deductions taken in the prior year and the standard deduction amount for the prior year, if the taxpayer was not precluded from taking the standard deduction in the prior year.
This holding applies to the recovery of any state or local tax, including state or local income tax and state or local real or personal property tax.
The IRS provided four examples in the revenue ruling to illustrate the interaction of the limit and the tax benefit rule. The IRS noted that the ruling has no impact on state or local tax refunds received in 2018 and reportable on 2018 returns taxpayers are filing this season.Allocation Of Refund Form
The AICPA requested guidance on this issue from the IRS in its June 2018 recommendations for 2018-2019 guidance.Allocation Refund Direct Service
— Sally P. Schreiber, J.D., (Sally.Schreiber@aicpa-cima.com) is a JofA senior editor.
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noun
*
1A particular time or instance of an event.‘on one occasion I stayed up until two in the morning’
*‘Holidays and other special occasions are marked with singing and dancing.’
*‘Theresa is already working on big celebrations to mark the special occasion in the history of the prominent Association.’
*‘Ceremonies marking many official occasions are held in the country’s churches.’
*‘There had been only one previous occasion when I actually had to appear in court.’
*‘Rovers boast an excellent record in the competition having reached the final on two previous occasions in recent years.’
*‘Here BBC Sport recalls the five previous occasions when the ultimate prize in European rugby has been on the line.’
*‘Actually, I could only remember one other occasion, months and months ago.’
*‘The accused testified and offered excuses in relation to her failure to comply on several other occasions during this period.’
*‘The meal followed and it proved to be a most enjoyable social occasion.’
*‘In 1979 Jimmy Carter used the occasion of the tenth anniversary of the lunar landing to push his energy program.’
*‘Holland has won the title five times while Pakistan emerged victorious on three occasions.’
*‘We took a while to find the right direction on two or three occasions.’
*‘On at least three difference occasions, Neil accepted the hospitality of his hosts.’
*‘His swimming marathon last summer was on the occasion of his 40th birthday.’
*‘Already, the industry has found sales touching the expected figures on four occasions.’
*‘On all four occasions, patients were transferred to other hospitals in the city.’
*‘Were the written words used by the defendant on an occasion of qualified privilege?’
*‘Not everything said or written on an occasion of qualified privilege is protected.’
*‘The trial of Mr Daly had been adjourned on a number of occasions in the past.’
*‘You have been before the courts on many occasions over the years.’
*1.1A special or noteworthy event, ceremony, or celebration.‘she was presented with a gold watch to mark the occasion’
*‘The name-giving ceremony is a formal occasion celebrated by feasting and drinking.’
*‘Mass will be celebrated to mark the occasion and the dinner and party will be held in the Anglers Rest Hotel in Headford.’
*‘In 1974 he was invited to address the US Congress on the occasion of the celebrations marking the American bicentennial.’
*‘Some cultures decorate to celebrate a festive occasion, others to mark membership in a group or tribe.’
*‘The ceremony was an occasion for considerable celebration, but Margaret was never crowned queen.’
*‘Her family and friends, along with staff at St. Joseph’s, had a right old party bash celebrating the occasion.’
*‘They marked the occasion with a celebration with family and neighbours.’
*‘Last week there were celebrations to mark the occasion with Mass being celebrated by the new Bishop of Galway.’
*‘Nobody is going to have that many special occasions in their life.’
*‘Another center of Shan life is the Buddhist monastery, where many occasions are celebrated.’
*‘They gave me a crystal wine glass to celebrate the momentous occasion.’
*‘To celebrate this momentous occasion, have all your neighbors get together for a good old fashioned barbeque.’
*‘He did this on several festive, campy occasions.’
*‘She was crowned at the end of a gala occasion on Sunday night.’
*‘Today, on the occasion of his 70th birthday, we wish him well.’
*‘The recent parish outing and day trip to West Mayo proved a very enjoyable occasion.’
*‘Weddings are important and costly occasions for celebration in both the Portuguese and Chinese communities.’
*‘The greatest occasion for celebration in a Kurd’s life is marriage.’
*‘Patel admitted he had initially been overawed by the occasion.’
*‘The entire set, costumes and props will also be flown to New York for the gala occasion.’social event, event, affair, function, celebration, party, ceremony, get-together, gatheringView synonyms
*1.2A suitable or opportune time for doing something.‘elections are an occasion for registering protest votes’
*‘Opportunity refers to the occasion suitable for or conducive to the behavior, including such factors as geography and time.’
*‘The occasion arose through the trip of the old people to Poppleton, given by Captain Grace, on the ‘River King’ a few weeks before.’
*‘Ms. Ayotte said she was prepared to issue a formal opinion to that effect if the occasion arose.’
*‘Thankfully, they are all still fit and well and could still turn on the style if the occasion arose.’
*‘I promised myself that I’d tell Karl as soon as the occasion arose.’
*‘Instead of passing the ball off every time, he took shots around the arch when the occasion arose.’
*‘Raf was a great playmate to have around and could be serious when the occasion arose.’
*‘Whenever the occasion arose to work in a different area or learn new skills, I welcomed it.’
*‘I reckon that birthdays are more suitable occasions for resolutions than New Year.’
*‘Having some occasions or opportunities to do so is always rewarding and inspiring.’
*‘The special occasion gave guests the opportunity to meet the director, singers and dancers of the world renown opera.’
*‘The occasion afforded staff the opportunity of paying tribute to a long-standing member of staff.’
*‘I struggle to recall those rare occasions when it has been invoked against a non-Arab state.’
*‘The Assembly used this rare occasion to chat about everything and nothing and just enjoy the time they spent together.’
*‘The controversy provided a rare occasion for agreement between the ruling right and opposition left.’
*‘I promised myself that I’d tell Karl as soon as the occasion arose.’
*‘At once, occasion arose for versions of reality to compete for public credibility.’
*‘Each situation is different, and must be assessed individually when the occasion arises.’
*‘The Bodhisattva can be represented as both male and female as need and occasion demand.’
*‘We addicts can always come up with suitable justifications when the occasion demands.’opportunity, opportune time, suitable time, right moment, chance, opening, windowView synonyms
*
2formal Reason; cause.with infinitive‘it’s the first time that I’ve had occasion to complain’
*‘Actually, there is no special occasion or reason to buy the stuff.’
*‘There may be occasion at work and reason at home, for you to lose your cool or balance but that’s not helpful so avoid extremes of any kind.’
*‘For the first thirty years of my academic career, I had no occasion and no reason to worry about sports.’
*‘About this time last year I had occasion to complain about the non-collection of my refuse bin.’
*‘I think you are somewhat reserved, but my daughter does not seem to mind your taciturnity, so I suppose I have no occasion to complain.’
*‘Our certainty, whether grounded in reason or miraculous signs, affords no occasion to trust.’
*‘I often have occasion to be reminded of the man who, as executive deputy mayor, was the face and the voice of Buffalo City.’reason, cause, call, grounds, justification, need, necessity, requirement, excuse, pretext, stimulus, inducement, provocation, motiveView synonyms
901.185 tweedehands auto’s van Duitsland te koop. Kopen tweedehands auto online. Wij helpen u bij het kiezen van de beste gebruikte auto op de automarkt. Our goal is to use every opportunity we have—no matter how small—to set change in motion. To be a force for good and a force for growth. For you, for the world, and for every generation to come.transitive verb[with object]formal
*
Cause (something)
*‘his death occasioned her much grief’
*‘Much sadness was occasioned by the sudden death of well known Claremorris chemist Sean O’Brien at the weekend.’
*‘Patrick was a popular and esteemed member of the local rural community and much sadness was occasioned by his death.’
*‘These rites control the pollution occasioned by death, and also usher the soul from one life to another.’
*‘His death occasioned an outpouring of condolences, mourning, and reflection.’
*‘Much sadness was occasioned around the area by the news of her sudden death.’
*‘Last year, the lights were not removed until March, occasioning embarrassment for both the Chamber and the Council.’
*‘But all in all, what with the weather and a degree of jadedness occasioned by a bit of sleep disturbance, it’s not been a thrilling day.’
*‘My examination of terms such as fraught and wrought has occasioned controversy.’
*‘The jury took two hours to convict him of assault occasioning actual bodily harm.’
*‘Patrick was a popular and esteemed member of the local rural community and much sadness was occasioned by his death.’
*‘In principle any losses occasioned thereby are recoverable however they may be characterised.’
*‘After a long delay occasioned by France, the treaty entered into force in 1985.’
*‘The loss occasioned by cancellation of hotel bookings and other expenses runs into the billions of shillings.’
*‘His death shortly afterwards occasioned louder public grief than that of Louis XV four years earlier.’
*‘His passing occasioned deep grief in his native Ballinrobe where he was deservedly held in very high regard.’
*‘In addition, she received an apology for any injustice occasioned to her.’
*‘Do you have to show that the error is one which occasions an injustice to your client?’
*‘The injustices occasioned by these institutions should, however, be confronted as an aspect of spiritual practice.’
*‘The father was charged with assault occasioning actual bodily harm, but was acquitted.’
*‘Johnston has been charged with malicious wounding and assault occasioning actual bodily harm.’cause, give rise to, bring about, result in, lead to, prompt, provoke, evoke, elicit, call forth, produce, create, arouse, make, make for, generate, engender, originate, effect, bring on, induce, precipitate, stir up, inspire, spark off, trigger, breedView synonymsOccasions DuitslandOccasion Duitsland ZoekenPhraseson occasion
*
Occasionally; from time to time.
*‘The three journalists who interviewed Putin for this book are pleasingly sassy on occasion.’
*‘Once there, Joe’s life became one of living in hostels or, on occasions, even sleeping rough.’
*‘My students used to ask on occasions whether they were different from my students in Czechoslovakia.’
*‘The Laois lads continued to dominate the exchanges but on occasions were guilty of some wayward passes.’
*‘Now bear in mind this is the same person who, on occasions, will spend hours on her make-up before she’ll set foot out the door.’
*‘A post as full-time director of the charity has meant that he has been on occasions unavailable for team selection.’
*‘Celebrity Melinda has also been persuaded to join him on occasions.’
*‘Early on, maybe, I was a little rusty on occasions, but I felt much better as the match went on.’
*‘We have lost by half a point and one point on occasions and probably have the most second places in Panorama.’
*‘His health is such that he turns blue on occasions and is unable to breathe.’take occasionarchaic
*
Make use of an opportunity to do something.‘I shall here take occasion to propose a second observation’
*‘I took occasion from thence to speak strongly to her, concerning the hand of God, and his design in all afflictions.’
*‘For I am possessed of a cat, surpassing in beauty, from whom I take occasion to bless Almighty God.’
*‘But sin, taking occasion by the Commandment, wrought in me all manner of concupiscence.’
*‘Here I must take occasion to tell you, there are five faults will be found in heaven with your best fruits.’
*‘The member does not even know the Standing Orders, and he should take occasion over the adjournment to read them.’rise to the occasion
*
Perform very well in response to a special situation or event.‘when it comes to the finals, they can rise to the occasion’
*‘As the event unfolded, Samuel rose to the occasion.’
*‘Nadia rose to the occasion, performing almost flawlessly.’
*‘Australians who came into the game with a ‘must win’ situation rose to the occasion in fine style and outplayed the Kiwis.’
*‘I also like the fact that he doesn’t just rise to the occasion in certain events that suit his style.’
*‘Along with the epic quality of the storytelling come performances that rise to the occasion.’
*‘The team rose to the occasion and responded magnificently.’
*‘Still this is a masterful performance by Andsnes who rises to the occasion quite wonderfully.’
*‘Many teens will rise to the occasion by taking on responsibilities and providing support for other family members.’
*‘There is some fine virtuoso writing here too, and the performers certainly rise to the occasion.’
*‘Morrisons staff in Keighley are fired up about their store’s latest improvement and hope customers will rise to the occasion.’Occasion Duitsland ImporterenOrigin
Late Middle English from Latin occasio(n-) ‘juncture, reason’, from occidere ‘go down, set’, from ob- ‘towards’ + cadere ‘to fall’.Occasion Auto DeutschlandBmw Occasion DuitslandAre You Learning English? Here Are Our Top English Tips
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*R Gplots Heatmap.2
*Gplots Heatmap.2 Example
*Gplots R Heatmap.2
*Gplots Heatmap.2 Colors
*Plots Heatmap
The first section of this page uses R to analyse an Acute lymphocytic leukemia (ALL) microarray dataset, producing a heatmap (with dendrograms) of genes differentially expressed between two types of leukemia.
There is a follow on page dealing with how to do this from Python using RPy.
Demonstrate the effect of row and column dendrogram options heatmap.2(x) ## default - dendrogram plotted and reordering done. Heatmap.2(x, dendrogram=’none’) ## no dendrogram plotted, but reordering done.
The original citation for the raw data is ’Gene expression profile of adult T-cell acute lymphocytic leukemia identifies distinct subsets of patients with different response to therapy and survival’ by Chiaretti et al. Blood 2004. (PMID: 14684422)
Demonstrate the effect of row and column dendrogram options heatmap.2(x) ## default - dendrogram plotted and reordering done. Heatmap.2(x, dendrogram=’none’) ## no dendrogram plotted, but. I’m visualizing a data set with the heatmap.2 function from the gplots package in R. Basically I’m performing a hierarchical clustering analysis on the original data, while forcing the heatmap to display a limited version of the data (between -3 and +3) to limit the effect of outliers on the appearance of the heatmap, while still retaining the. This articles describes how to create and customize an interactive heatmap in R using the heatmaply R package, which is based on the ggplot2 and plotly.js engine.
The analysis is a ’step by step’ recipe based on this paper, Bioconductor: open software development for computational biology and bioinformatics, Gentleman et al. 2004. Their Figure 2 Heatmap, which we recreate, is reproduced here:Heatmaps from R
Assuming you have a recent version of R (from The R Project) and BioConductor (see Windows XP installation instructions), the example dataset can be downloaded as the BioConductor ALL package.
You should be able to install this from within R as follows:
Alternatively, you can download the package by hand from here or here.
If you are using Windows, download ALL_1.0.2.zip (or similar) and save it. Then from within the R program, use the menu option ’Packages’, ’Install package(s) from local zip files...’ and select the ZIP file.
On Linux, download ALL_1.0.2.tar.gz (or similar) and use sudo R CMD INSTALL ALL_1.0.2.tar.gz at the command prompt. With that out of the way, you should be able to start R and load this package with the library and data commands:
If you inspect the resulting ALL variable, it contains 128 samples with 12625 genes, and associated phenotypic data.
We can looks at the results of molecular biology testing for the 128 samples:
Ignoring the samples which came back negative on this test (NEG), most have been classified as having a translocation between chromosomes 9 and 22 (BCR/ABL), or a translocation between chromosomes 4 and 11 (ALL1/AF4).
For the purposes of this example, we are only interested in these two subgroups, so we will create a filtered version of the dataset using this as a selection criteria:
The resulting variable, eset, contains just 47 samples - each with the full 12,625 gene expression levels.
This is far too much data to draw a heatmap with, but we can do one for the first 100 genes as follows:
According to the BioConductor paper we are following, the next step in the analysis was to use the lmFit function (from the limma package) to look for genes differentially expressed between the two groups. The fitted model object is further processed by the eBayes function to produce empirical Bayes test statistics for each gene, including moderated t-statistics, p-values and log-odds of differential expression.
If the limma package isn’t installed, you’ll need to install it first:
We can now reproduce Figure 1 from the paper.
The leftmost numbers are row indices, ID is the Affymetrix HGU95av2 accession number, M is the log ratio of expression, A is the log average expression, t the moderated t-statistic, and B is the log odds of differential expression.
Next, we select those genes that have adjusted p-values below 0.05, using a very stringent Holm method to select a small number (165) of genes.
The variable esetSel has data on (only) 165 genes for all 47 samples . We can easily produce a heatmap as follows (in R-2.1.1 this defaults to a yellow/red ’heat’ colour scheme): If you have the topographical colours installed (yellow-green-blue), you can do this:
This is getting very close to Gentleman et al.’s Figure 2, except they have added a red/blue banner across the top to really emphasize how the hierarchical clustering has correctly split the data into the two groups (10 and 37 patients).
To do that, we can use the heatmap function’s optional argument of ColSideColors. I created a small function to map the eselSet$mol.biol values to red (#FF0000) and blue (#0000FF), which we can apply to each of the molecular biology results to get a matching list of colours for our columns:
Looks pretty close now, doesn’t it:
To recap, this is ’all’ we needed to type into R to achieve this: Heatmaps in R - More Options
One subtle point in the previous examples is that the heatmap function has automatically scaled the colours for each row (i.e. each gene has been individually normalised across patients). This can be disabled using scale=’none’, which you might want to do if you have already done your own normalisation (or this may not be appropriate for your data):
heatmap(exprs(esetSel), col=topo.colors(75), scale=’none’, ColSideColors=patientcolors, cexRow=0.5)
You might also have noticed in the above snippet, that I have shrunk the row captions which were so big they overlapped each other. The relevant options are cexRow and cexCol.
So far so good - but what if you wanted a key to the colours shown? The heatmap function doesn’t offer this, but the good news is that heatmap.2 from the gplots library does. In fact, it offers a lot of other features, many of which I deliberately turn off in the following example:
By default, heatmap.2 will also show a trace on each data point (removed this with trace=’none’). If you ask for a key (using key=TRUE) this function will actually give you a combined ’color key and histogram’, but that can be overridden (with density.info=’none’).
Don’t like the colour scheme? Try using the functions bluered/redblue for a red-white-blue spread, or redgreen/greenred for the red-black-green colour scheme often used with two-colour microarrays: Heatmaps from Python
So, how can we do that from within Python? One way is using RPy (R from Python), and this is discussed on this page.
P.S. If you want to use heatmap.2 from within python using RPy, use the syntax heatmap_2 due to the differences in how R and Python handle full stops and underscores. What about other microarray data?
Well, I have also documented how you can load NCBI GEO SOFT files into R as a BioConductor expression set object. As long as you can get your data into R as a matrix or data frame, converting it into an exprSet shouldn’t be too hard.
This was all written and tested using Windows XP and R-2.1.1, but it should all work on other platforms.
The first section of this page uses R to analyse an Acute lymphocytic leukemia (ALL) microarray dataset, producing a heatmap (with dendrograms) of genes differentially expressed between two types of leukemia.
There is a follow on page dealing with how to do this from Python using RPy.
The original citation for the raw data is ’Gene expression profile of adult T-cell acute lymphocytic leukemia identifies distinct subsets of patients with different response to therapy and survival’ by Chiaretti et al. Blood 2004. (PMID: 14684422)
The analysis is a ’step by step’ recipe based on this paper, Bioconductor: open software development for computational biology and bioinformatics, Gentleman et al. 2004. Their Figure 2 Heatmap, which we recreate, is reproduced here:Heatmaps from R
Assuming you have a recent version of R (from The R Project) and BioConductor (see Windows XP installation instructions), the example dataset can be downloaded as the BioConductor ALL package.
You should be able to install this from within R as follows:
Alternatively, you can download the package by hand from here or here.
If you are using Windows, download ALL_1.0.2.zip (or similar) and save it. Then from within the R program, use the menu option ’Packages’, ’Install package(s) from local zip files...’ and select the ZIP file.
On Linux, download ALL_1.0.2.tar.gz (or similar) and use sudo R CMD INSTALL ALL_1.0.2.tar.gz at the command prompt. With that out of the way, you should be able to start R and load this package with the library and data commands: R Gplots Heatmap.2
If you inspect the resulting ALL variable, it contains 128 samples with 12625 genes, and associated phenotypic data.
We can looks at the results of molecular biology testing for the 128 samples: Gplots Heatmap.2 Example
Ignoring the samples which came back negative on this test (NEG), most have been classified as having a translocation between chromosomes 9 and 22 (BCR/ABL), or a translocation between chromosomes 4 and 11 (ALL1/AF4).
For the purposes of this example, we are only interested in these two subgroups, so we will create a filtered version of the dataset using this as a selection criteria:
The resulting variable, eset, contains just 47 samples - each with the full 12,625 gene expression levels.
This is far too much data to draw a heatmap with, but we can do one for the first 100 genes as follows:
According to the BioConductor paper we are following, the next step in the analysis was to use the lmFit function (from the limma package) to look for genes differentially expressed between the two groups. The fitted model object is further processed by the eBayes function to produce empirical Bayes test statistics for each gene, including moderated t-statistics, p-values and log-odds of differential expression.
If the limma package isn’t installed, you’ll need to install it first:
We can now reproduce Figure 1 from the paper.
The leftmost numbers are row indices, ID is the Affymetrix HGU95av2 accession number, M is the log ratio of expression, A is the log average expression, t the moderated t-statistic, and B is the log odds of differential expression.
Next, we select those genes that have adjusted p-values below 0.05, using a very stringent Holm method to select a small number (165) of genes.
The variable esetSel has data on (only) 165 genes for all 47 samples . We can easily produce a heatmap as follows (in R-2.1.1 this defaults to a yellow/red ’heat’ colour scheme): Gplots R Heatmap.2 If you have the topographical colours installed (yellow-green-blue), you can do this:
This is getting very close to Gentleman et al.’s Figure 2, except they have added a red/blue banner across the top to really emphasize how the hierarchical clustering has correctly split the data into the two groups (10 and 37 patients).
To do that, we can use the heatmap function’s optional argument of ColSideColors. I created a small function to map the eselSet$mol.biol values to red (#FF0000) and blue (#0000FF), which we can apply to each of the molecular biology results to get a matching list of colours for our columns:
Looks pretty close now, doesn’t it:
To recap, this is ’all’ we needed to type into R to achieve this: Heatmaps in R - More Options
One subtle point in the previous examples is that the heatmap function has automatically scaled the colours for each row (i.e. each gene has been individually normalised across patients). This can be disabled using scale=’none’, which you might want to do if you have already done your own normalisation (or this may not be appropriate for your data):
heatmap(exprs(esetSel), col=topo.colors(75), scale=’none’, ColSideColors=patientcolors, cexRow=0.5)
You might also have noticed in the above snippet, that I have shrunk the row captions which were so big they overlapped each other. The relevant options are cexRow and cexCol.
So far so good - but what if you wanted a key to the colours shown? The heatmap function doesn’t offer this, but the good news is that heatmap.2 from the gplots library does. In fact, it offers a lot of other features, many of which I deliberately turn off in the following example:
By default, heatmap.2 will also show a trace on each data point (removed this with trace=’none’). If you ask for a key (using key=TRUE) this function will actually give you a combined ’color key and histogram’, but that can be overridden (with density.info=’none’).
Don’t like the colour scheme? Try using the functions bluered/redblue for a red-white-blue spread, or redgreen/greenred for the red-black-green colour scheme often used with two-colour microarrays: Heatmaps from Python
So, how can we do that from within Python? One way is using RPy (R from Python), and this is discussed on this page.
P.S. If you want to use heatmap.2 from within python using RPy, use the syntax heatmap_2 due to the differences in how R and Python handle full stops and underscores. What about other microarray data?Gplots Heatmap.2 Colors
Well, I have also documented how you can load NCBI GEO SOFT files into R as a BioConductor expression set object. As long as you can get your data into R as a matrix or data frame, converting it into an exprSet shouldn’t be too hard. Plots Heatmap
This was all written and tested using Windows XP and R-2.1.1, but it should all work on other platforms.
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*Plot Legend Matlab
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*Plot Legends Of The Fall
*Plot Legend
The sun hasn’t set on Day and June. Marie Lu’s bestselling dystopian Legend Trilogy, which seemingly concluded with Champion in 2013, is getting a fourth and final book.
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*Definition of Transfer Functions in Matlab. The transfer function of the LTI system is the ratio of the Laplace transform of output to the Laplace transform of input of the system by assuming all the initial conditions are zero.
PlotLegends is an option for plot functions that specifies what legends to use.
Marie Lu revealed the title of Legend Book Four on Twitter Monday morning. The book will be called Rebel and will take place 10 years following the events of Champion, according to The Washington Post.
Related:Here’s what happens in Legend Book 4, Rebel (Discussion — SPOILERS!!)
“I can’t believe I get to share this with you all,” Lu shared in a tweet.
https://twitter.com/Marie_Lu/status/1092468432296005633
Unlike the original trilogy, Rebel looks to explore Day’s younger brother, Eden. That’s not to say Day and June won’t be players in this final story. The book’s synopsis, also released on Monday, suggests that we’ll be following all three characters.
Read the synopsis below:
Eden Wing is a brilliant student. He’s about to graduate a year early from Ross University of the Sciences, with honors, and already has an internship lined up back in the Republic. But most people don’t introduce Eden this way. Instead, they say, “This is Eden, Daniel Wing’s younger brother.”Plot Legend Matlab
Ten years ago, Eden’s brother Daniel was known as Day, the boy from the streets who led a revolution that saved the Republic of America. His name was spray-painted on walls, his profile splashed on both rebel pamphlets and wanted posters. He went from being a hunted criminal to a national hero in less than a year.
Day has spent the past decade piecing together his memory of his time in the Republic, pretending to enjoy life in Antarctica’s capital, Ross City , and quietly hiding out from the world—even if it’s meant giving up June, the great love of his life. As long as he can keep his little brother safe, that’s all that matters . . .Plot Legend Of Tarzan Movie
But Eden isn’t safe. As the two brothers struggle to accept who they’ve each become, they grow more distant from one another than they’ve ever been. Eden finds himself drawn deeper and deeper into Ross City’s dark side, until even his legendary brother can’t save him. At least not on his own.
When June suddenly steps back into Day’s life, rekindling the flames of their romance, the pair team up to do whatever it takes to rescue Eden. But like the rest of the world, they may have underestimated Eden—what he’s capable of, where his loyalties lie, and how hard he’s willing to fight for what he believes.
With unmatched suspense and her signature cinematic storytelling, #1 New York Times bestselling author Marie Lu plunges readers back into the unforgettable world of Legend—for the most thrilling adventure yet. A truly grand finale.
News of a fourth Legend novel circulated the YA community last fall when The Washington Post announced that Lu had signed a multimillion-dollar book deal with publisher Roaring Book Press (an imprint of Macmillan Children’s Publishing Group). The deal included a fourth Legend novel as well as two books in an upcoming science-fiction/fantasy YA series titled Skyhunter.
Lu first rose to prominence in the YA community with her Legend novels, the first of which was published in 2011. Since then, she’s completed two other YA series: The Young Elites and Warcross.Plot Legends Of The Fall
Rebel is set to hit shelves in October. Fans can already pre-order the book.Plot Legend010401
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